| 대식세포가 β-tricalcium Phosphate 뼈이식제의 생분해에 미치는 영향 |
| 김영희, Anirban Jyoti, 변인선, 오익현1, 민영기2, 양훈모2, 이병택3, 송호연 |
순천향대학교 의과대학 미생물학교실
1한국생산기술연구원 광주연구센터
2순천향대학교 의과대학 생리학교실
3순천향대학교 의과대학 의공학교실 |
| Effects of Macrophage on Biodegradation of β-tricalcium Phosphate Bone Graft Substitute |
| Young-Hee Kim, Anirban Jyoti, In-Sun Byun, Ik-Hyun Oh1, Young-Ki Min2, Hun-Mo Yang2, Byong-Taek Lee3, Ho-Yeon Song |
Department of Microbiology, School of Medicine, Soonchunhyang University
1Korea Institute of Industrial Technology(KlTECH), Gwangju Research Center
2Department of Physiology, School of Medicine, Soonchunhyang University
3Department of Biomedical Engineering & Materials, School of Medicine, Soonchunhyang University
4 ᘀ ऐ 돀탍ﶖ⨀ 塨 |
|
|
|
|
|
| ABSTRACT |
|
Various calcium phosphate bioceramics are distinguished by their excellent biocompatibility and osteoconductivity. Especially, the exceptional biodegradability of $beta$-TCP makes it a bone graft substitute of choice in many clinical applications. The activation of osteoclasts, differentiated from macrophage precursor cells, trigger a cell-mediated resorption mechanism that renders $beta$-TCP biodegradable. Based on this evidence, we studied the biodegradation process of granular-type $beta$-TCP bone graft substitute through in vitro and in vivo studies. Raw 264.7 cells treated with RANKL and M-CSF differentiated into osteoclasts with macrophage-like properties, as observed with TRAP stain. These osteoclasts were cultured with $beta$-TCP nano powders synthesized by microwave-assisted process. We confirmed the phagocytosis of osteoclasts by observing $beta$-TCP particles in their phagosomes via electron microscopy. No damage to the osteoclasts during phagocytosis was observed, nor did the $beta$-TCP powders show any sign of cytotoxicity. We also observed the histological changes in subcutaneous tissues of rats implanted with granule-type $beta$-TCP synthesized by fibrous monolithic process. The $beta$-TCP bone graft substitute was well surrounded with fibrous tissue, and 4 months after implantation, 60% of its mass had been biodegraded. Also, histological findings via H&E stain showed a higher level of infiltration of lymphocytes as well as macrophages around the granule-type $beta$-TCP. From the results, we have concluded that macrophages play an important role in the biodegradation process of $beta$-TCP bone graft substitutes. |
| Key words:
Bone graft substitute$\beta$-TCP, Biodegradation, Macrophage |
|
|
|
PDF Links
Full text via DOI
Download Citation 
